Table 1.
Primers used in molecular confirmation study
| Primer name | Primer sequences | Annealing temperature | Amplification size | Purpose/Reference |
|---|---|---|---|---|
| VMP 23-1-F | 5′ CTTTTGGGTCATCCTRGA 3′ | 48°C | 1,051 bp | Identification (Bauernfeind et al., 1998) |
| MP 23-2-R | 5′ TCCTACCATGCGAGACT 3′ | |||
| BPTTS1-F | 5′CGTCTCTATACTGTCGAGCAATCG 3′ | 58°C | 548 bp | Identification (Novak et al., 2006) |
| BPTTS1-R | 5′ CGTGCACACCGGTCAGTATC 3′ | |||
| F8 | 5′ AGTTTGATCCTGGCTCAG 3′ | 50°C | 1,488 bp | 16S sequencing (Gee et al., 2003) |
| R1492 | 5′ ACCTTGTTACGACTT 3′ | |||
| fli C-F | 5′ CTCGGATCCAACAGCAAC 3′ | 52°C | 1,167 bp | PCR-RFLP (Primer designed) |
| fli C-R | R- 5′ TATTGCAGGTACCTTCAG 3′ |