Table 2.
Results of CCS and cluster analyses of the V1R I and V1R IX repertoires of two individuals of M. murinus
| M. murinus1 | M. murinus2 | |
|---|---|---|
| Raw CCS reads | 29,739 | 32,837 |
| Post quality filter | 16,914 | 17,174 |
| V1RI CCS reads | 12,625 | 11,814 |
| V1RIX CCS reads | 4,289 | 5,360 |
| V1RI Clusters (≥7×) | 106 | 114 |
| V1RIX Clusters (≥7×) | 61 | 85 |
| CCS V1RI loci (98 to 99%) | 22–28 | 20–27 |
| CCS V1RIX loci (98 to 99%) | 20–24 | 20–28 |
| Estimated V1RI repertoire | 34 (30)1 | 36 |
| Estimated V1RIX repertoire | 32 (19)1 | 28 |
PacBio CCS V1R subfamily loci are estimates based on 98% to 99% genetic similarity thresholds (see Methods). Final repertoire estimates are based on cluster analyses of all available V1RI and V1RIX data (e.g. Young et al. [18], Yoder et al. [29], and this study). Chimera detection results are presented in Additional file 1: Figure S2. 1Number of functional genes mined from the draft M. murinus genome by Young et al. [18].