Table 3.
Polymorphism statistics of DNA sequence data for the V1R subfamilies examined herein
| M. murinus1 | N | Fragment size (bp) | S | k | π | θ W | Syn | NonSyn |
|---|---|---|---|---|---|---|---|---|
| V1RI (CCS) | 29 | 678 | 223 | 55.75 | 0.083 | 0.084 | 160.78 | 508.22 |
| V1RI (genome) | 30 | 678 | 225 | 57.60 | 0.086 | 0.084 | 160.17 | 508.83 |
| V1RIX (CCS) | 24 | 750 | 199 | 42.22 | 0.056 | 0.071 | 186.69 | 560.31 |
| V1RIX (genome) | 19 | 750 | 181 | 42.88 | 0.057 | 0.069 | 186.75 | 563.25 |
| M. murinus 2 | ||||||||
| V1RI (CCS) | 27 | 678 | 228 | 54.83 | 0.082 | 0.088 | 160.95 | 508.05 |
| V1RI (Sanger) | 43 | 678 | 271 | 56.96 | 0.085 | 0.093 | 161.01 | 507.99 |
| V1RIX (CCS) | 30 | 750 | 214 | 45.69 | 0.061 | 0.072 | 187.28 | 562.72 |
Number of sequences per methodology (N), number of segregating sites (S), average number of nucleotide differences between sequences (k), mean nucleotide diversity (π), Watterson’s estimator of mutation rate (θW), raw number of synonymous (Syn) and nonsynonomous (NonSyn) mutations. CCS = PacBio Circular Consensus Sequencing; genome = sequences mined from the M. murinus draft genome [18]; Sanger = sequences originating from Yoder et al. [29].