Fig. 2.
Western blotting analysis of BRAF expression, MEK phosphorylation, and ERK phosphorylation in cell clones stably transfected with various siRNAs. A, Clones derived from KAT10 cells. B, Clones derived from NPA cells. Several different individual cell clones for each type of specific or control siRNA were selected and identified, all showing a dramatic decrease in BRAF protein, MEK phosphorylation, and ERK phosphorylation in specific siRNA clones compared with nonspecific siRNA clones. p-MEK, Phosphorylated MEK; t-MEK, total MEK protein.
