Fig 5.

Western blot of SpSsp1 incubated with serum obtained from trout and salmon from fish farms in the UK. The same amount of enriched SpSsp1 was loaded in each lane and run on an SDS-PAGE gel, blotted and a Western blot was performed with sera of six individual fish from several fish farms. (A) Serum from a random salmon harvested from a farm in Scotland. (B–F) Serum from rainbow trout obtained from three independent trout farms in the UK (B–E). (E) A severely wounded trout, which did not show any visual symptoms of saprolegniosis. (F) Rainbow trout maintained in the fresh water aquarium facilities at the University of Aberdeen. The X-ray film was exposed to blots (A–D) and (F) for 2 min and blot (E) for 20 s. There is no recognition of recombinant SpSsp1 protein by sera samples from the salmon (A), and the trout of two fish farms (B & C), weak recognition of protein by sera from the third fish farm (D) and the aquarium facilities at the University of Aberdeen (F). Serum of the injured fish from farm (E) showed a strong response towards SpSsp1.