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. 2014 Jul;118(7):621–629. doi: 10.1016/j.funbio.2014.01.011

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© 2014 The British Mycological Society. Elsevier Ltd. All rights reserved.

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Fig 3 — Percentage of non-silencing obtained on individual lines using RNAi. mRNA from lines SpTyr-dsRNA treated and gfp-dsRNA was extracted as described in Material and Methods. Gene expression levels of individual lines, after RNAi assay, were determined by qPCR analysis and corrected for exogenously added mRNA of b-tubulin (housekeeping gene). Its value is expressed in percentage of non-silencing. The percentage was calculated considering the control, gfp-dsRNA treated lines, as 100 % non-silenced. Note that SpTyr-dsRNA individual lines Tyr1 to Tyr5 present the higher level of silencing ranging between 60 % and 38 %. The error bars represent the standard error within three technical replicates.