Figure 6.

Other RTK inhibitors targeting VEGFRs facilitate the self-renewal of mESCs and the generation of iPSCs. (A) E14 cells were cultured in LIF-free mES media containing sunitinib or other inhibitors at the indicated concentrations for 3 days. The initial cell density was 1 000 cells/well in 96-well plate. The percent of AP⁺ (undifferentiated) and AP⁻ (differentiated) colonies were calculated. 2i culture was used as a positive control. (B) MEFs transduced with Yamanaka 4 factors were treated with sunitinib or the indicated inhibitors (added from day 3 to day 14) and GFP⁺ colonies were counted on day 14. VPA (1 mM, added from day 3 to day 8) treatment was used as a positive control. Data are mean ± SEM (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs vehicle control.