Table 1.
rtPCR results obtained from the enrichment protocols evaluated in experiment 1 for Y. pseudotuberculosis and Y. enterocolitica
| Enrichment protocol |
Y. enterocolitica
rtPCR |
Y. pseudotuberculosis
rtPCR |
||||
|---|---|---|---|---|---|---|
| Pos (Ct ≤) | Pos (Ct >) | Neg. | Pos (Ct ≤) | Pos (Ct >) | Neg. | |
| CASO |
5 |
2 |
1 |
4 |
0 |
0 |
| PSB |
3 |
5 |
0 |
0 |
4 |
0 |
| ITC |
5 |
2 |
1 |
0 |
3 |
1 |
| PBS1 |
5 |
2 |
1 |
0 |
4 |
0 |
| PBS2 |
7 |
1 |
0 |
4 |
0 |
0 |
| PBS3 |
6 |
1 |
1 |
4 |
0 |
0 |
| PBS4 |
5 |
3 |
0 |
4 |
0 |
0 |
| PBSMSB |
5 |
3 |
0 |
4 |
0 |
0 |
| PBSSSB | 6 | 1 | 1 | 3 | 1 | 0 |
Pos (Ct ≤): rtPCR positive result after enrichment with Ct value minor or similar (±0.5) than Ct values from direct rtPCR.
Pos (Ct >): rtPCR positive result after enrichment with Ct value major than Ct values from direct rtPCR.
Neg: rtPCR negative result.