. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: Mol Microbiol. 2014 Aug 6;93(5):1026–1042. doi: 10.1111/mmi.12715

Table 3.

nilD is required for X. nematophila colonization of S. anatoliense and S. websteri nematodes.

Bacterial Strain	attTn7 locus insertion^a	Average CFU/IJ ± standard deviation (n=3)
Bacterial Strain	attTn7 locus insertion^a	S. carpocapsae	S. anatoliense	S. websteri
XnSc 081	none	42.07 ± 11.01^A	34.40 ± 4.12^A	26.31 ± 6.20^A
XnSc 081 nilD6::Tn5	none	0.10 ± 0.04^B	0.36 ± 0.06^B	0.12 ± 0.15^B
XnSc 081 nilD6::Tn5	nilD-Sc	54.40 ± 7.95^A	38.60 ± 4.77^A,C	44.10 ± 7.03^A,C
XnSc 081 nilD6::Tn5	nilD-Sa	1.09 ± 0.24^B	1.08 ± 0.40^B	0.45 ± 0.23^B
XnSc 081 nilD6::Tn5	nilD-Sw	0.03 ± 0.02^B	0.25 ± 0.17^B	0.02 ± 0.14^B
XnSa	none	77.90 ± 16.58^A,C	59.40 ± 4.13^C	55.50 ± 16.61^C,D
XnSw	none	63.55 ± 12.97^A,C	28.45 ± 6.66^A	74.57 ± 4.74^D

A Tn7 transposon carrying nilD loci from XnSc (nilD-Sc), XnSa (nilD-Sa), or XnSw (nilD-Sw) was integrated at the attTn7 locus. Different letters indicate significant differences between bacterial strains for colonization within each nematode species: p<0.05 using one-way repeated measures ANOVA (Prism v2.0, GraphPad, La Jolla, CA) with Tukey’s post-test. Colonization levels achieved by each bacterial strain in the three nematode species were not significantly different except XnSw for which colonization of S. anatoliense nematodes was significantly lower than those of the other two nematode species (not shown).