Figure. 2.

Normal ear tissue from B6 (Taconic; left) and MRL/MpJ (JAX; right) 8-week-old female mice were stained with anti-MPO antibody (red) and DAPI (blue). The staining pattern was consistent with toluidene blue staining for mast cells (data not shown). The level of staining was quantified and shown in the lower panel, indicating that MRL showed approximately threefold more staining than B6. Image analysis was carried out using an Olympus AX70 fluorescent microscope and SPOT camera with bounded software. The specific signal was measured by the ImageJ v1.46f software, provided by the NIH. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound