FIG. 4.

Induction of intracellular reactive oxygen species (ROS) generation in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. Monitoring of the ROS production with DCF (A) in MGC803 cells; fluorescence intensity (FI) was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, ^asignificantly different compared with the control group; ^bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf