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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Nat Struct Biol. 2003 Nov 9;10(12):1011–1018. doi: 10.1038/nsb1009

Figure 4.

Figure 4

Location of λ3 inside reovirus λ1 shell. (a) Space-filling representation of the inner surface of the λ1 shell viewed along a five-fold axis (white star). The five identical λ1-A subunits (those closest to the five-fold axis) are highlighted in yellow (A1), blue (A2), brown (A3), green (A4) and cyan (A5), and all five λ1-B subunits are shown in violet. Potential pores at the interfaces between neighboring λ1-A subunits are indicated by small red circles (shown also in c). The red line marks the position of a plane, ~6 Å from the five-fold axis, to one side of which over half of the pseudo atomic model of the reovirion was removed for the illustrations in e and f. (b) Same as a but with space-filling model of λ3 (red) added to show its position relative to the λ1 shell’s inner surface. The white arrow indicates the view direction in the cutaway illustrations in e and f. (c) Same as a but rendered to show the electrostatic surface potential of the λ1 shell inner wall. The λ3 footprint (black lines) outlines regions in λ1 within 5 Å of λ3 residues. (d) Electrostatic surface potential of λ3 overlaid with the footprint shown in c. The λ3 molecule (at right) and an enantiomeric version of it (at left) are viewed in a direction facing the mRNA exit channel and the surface that contacts the λ1 shell. The λ3 contact surface mainly consists of basic and neutral patches that seem complementary to the neutral and slightly acidic λ1 footprint seen in c. The λ3 enantiomer is included to help visualize the contact surface complementarity. (e) Shaded-surface, cutaway view of the pseudo atomic model of the reovirion, oriented with a five-fold axis vertical (red line) shows the arrangement of the globular λ3 molecules (red) on the inner surface of the λ1 shell. Most or all of the λ1-A1, -B2, -A2, -B3 and -A3 subunits, and small portions of the λ1-B1, -A5, -A4 and -B4 subunits, have been cut away. (f) Enlarged view of region outlined in e with λ2 in yellow, λ1 in blue and λ3 in red. Scale bars are labeled; bar in d also applies to a–c.