Table 2. Fitting the Second Dimer into the CryoEM Map after All Pixels Occupied by the First Dimer Were Set to Zero.

Fit	Sumf	Clash	–Den	Theta 1	Cent (x, y, z)	D1, D2, D3	D4, D5, D6
down +	48.3	20.0	57.0	204.5	48.8, −33.5, 221.6	44.2, 52.3, 51.8	51.3, 47.3, 40.9
down−	43.8	2026.0	105.0	345.2	87.5, −25.2, 210.8	39.1, 47.3, 43.1	49.2, 47.1, 30.8

Abbreviations are as in Table 1. The dimer 2-fold was placed radially passing through the point cent (x, y, z). D1, D2, D3 refer to the fits of the three domains in the first monomer, and D4, D5, D6 refer to the domain fits in the second monomer. These are at quasiequivalent positions in the map and have nonequivalent environments.