. Author manuscript; available in PMC: 2014 Sep 3.

Published in final edited form as: Nat Struct Biol. 2001 Oct;8(10):874–878. doi: 10.1038/nsb1001-874

Table 1.

Statistics of the cryo-EM reconstructions

	CVB3–full length CAR complex	CVB3–D1D2 complex	CVB3
Number of micrographs	26	18	9
Incubation time (min)	45	110	–
Defocus¹ (μm)	1.5–4.6	1.9–4.8	2.2–5.4
Magnification	61,000	45,000	61,000
Dose (e⁻ Å⁻²)	21.6	17.3	15.5
Number of particles
Selected	635	1,110	297
Total	1,565	2,637	997
Correlation coefficient²	0.386	0.441	0.491
Resolution³ (Å)	22	25	22

Determined from phase contrast transfer function of the microscope.

Real-space correlation coefficient (CC) for selected particles, CC = Σ ((rρ_i)(rρ_m) − <rρ_i><rρ_m>)/(Σ((rρ_i)2 − <rρ_i>2)Σ((rρ_m)2 − <rρ_m>2))1/2, where ρ_i is the electron density of the boxed cryo-EM image, ρ_m is the electron density of the model projection and r is the radius of the corresponding density point, which assures proper weighting of the densities. The angle brackets indicate mean values.

Resolution at which the correlation between two independent three-dimensional reconstructions falls below 0.5.