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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Biotechnol. 2014 May 18;32(6):554–561. doi: 10.1038/nbt.2915

Figure 4. Wnt manipulation allows for selective differentiation of primitive or definitive hematopoietic progenitors.

Figure 4

(A) Schematic for undisrupted embryoid body differentiation. hPSC are differentiated with Wnt activation or inhibition between days 2 and 3 of differentiation, followed by VEGF and cytokine supplementation until day 9, as in (1A). (B) Colony-forming progenitor potential of day 9 differentiation cultures, as in (A). n ≥ 5. (Mean ± SEM). Student’s t-test, relative to DMSO treatment * p ≤ 0.05 ** p ≤ 0.01. (C) Representative CD34 and CD43 expression of day 9 differentiation cultures, as in (A). (D) CD34+CD43− cells, as in (A) were analyzed for T cell potential after 28+ days OP9-DL4 co-culture. (E) Summary of hematopoietic potential following Wnt manipulation between days 2 and 3 of differentiation. CHIR99021 treatment prevents the specification of the primitive hematopoietic program while specifying the definitive program, while IWP2 prevents the specification of the definitive hematopoietic program while specifying the primitive hematopoietic program.