Fig. 4.

NS19504 increases BK currents in freshly isolated single USBM cells. (A) Representative whole-cell currents in response to 200-millisecond voltage ramps from −100 to +50 mV (insert in top) before (saline) and in the presence of NS19504 in the concentrations indicated to the right of the traces. BK channel inhibitor paxilline (1 µM, Pax) added at the end of experiment eliminates the effect of NS19504 and decreases the currents below the control (saline) amplitude. (B) Summary of relative to control percent increase of whole-cell BK currents by 0.32, 1.0, and 3.2 µM NS19504 measured at +50 mV. BK currents were obtained by subtraction of the currents in the presence of paxilline (1 µM) from control currents and those in the presence of NS19504. Data are means ± S.E. (n = 7– 8; *P < 0.05, one-way ANOVA).