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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Am J Physiol Cell Physiol. 2005 Aug;289(2):C233–C245. doi: 10.1152/ajpcell.00613.2004

Figure 1. Electroporation mediated gene transfer to skeletal muscle.

Figure 1

Balb/c mice were anesthetized and 50 μg of pEGFP-N1 (BD Clontech, Palo Alto CA) in 100 μl of saline was injected into the right and left quadriceps. Immediately following injection, a series of eight 10 msec pulses at 200 V/cm were delivered to the muscles of right leg (B); the left leg received DNA but was not electroporated. Twenty-four hours later, both muscles were exposed and photographed using a fluorescence dissecting microscope. As can be seen, robust gene expression in skeletal muscle is detected using electroporation.