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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Exp Cell Res. 1999 Dec 15;253(2):713–722. doi: 10.1006/excr.1999.4716

Figure 5. Nuclear import of plasmid DNA is rate limiting for gene expression at early times after microinjection.

Figure 5

pGFP was microinjected into the nucleus or cytoplasm of TC7 cells at the indicated copy number per cell and assayed for gene expression at (A) 4 and 8 hours, and (B) 24 hours post-injection. Between 100 and 150 cells were injected for each concentration shown. The results shown (A and B) are representative of 4 similar experiments.

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