Table 1.
Nuclear localization of plasmids.
| Subcellular Localization1 | |||
|---|---|---|---|
| Plasmid | Nucleus | Cytoplasm and Nucleus | Cytoplasm |
| SV40 (Complete Genome) | 82 ± 12 | 6 ± 2 | 12 ± 8 |
| pBR322 (No SV40) | 0 ± 0 | 0 ± 0 | 100 ± 0 |
| pOR-MCS (No SV40) | 0 ± 0 | 0 ± 0 | 100 ± 0 |
| pOR(5171-300) | 58 ± 4 | 22 ± 5 | 20 ± 9 |
| pOR(5171-43) | 8 ± 3 | 18 ± 1 | 74 ± 3 |
| pOR(5171-160) | 28 ± 11 | 27 ± 15 | 45 ± 5 |
| pOR(37-160) | 57 ± 3 | 21 ± 13 | 22 ± 10 |
| pOR(37-300) | 57 ± 6 | 20 ± 8 | 23 ± 2 |
| pOR(128-206) | 56 ± 3 | 16 ± 4 | 28 ± 5 |
| pOR(200-300) | 58 ± 7 | 20 ± 8 | 22 ± 15 |
| pOR(5208-43)20C | 8 ± 12 | 14 ± 8 | 77 ± 20 |
| pOR(5208-43)5215G | 6 ± 8 | 21 ± 11 | 73 ± 15 |
1
After cytoplasmic microinjection, cells were incubated for 8 hours, and scored for the subcellular location of the injected DNA by in situ hybridization. Cells were classified by either nuclear (≥80% nuclear signal), cytoplasmic and nuclear (≤80% nuclear signal), or cytoplasmic (0% nuclear) and tabulated as a percentage of cells with detectable signal. The results are the average (± st. dev.) of 4 experiments with between 50 and 100 cells visualized for each DNA.