Fig. 2.

Western blot analysis of α-Bgtx–purified nAChRs prepared from human basal forebrain and cerebellum. α-Bgtx–binding nAChRs were purified from the same volume of 2% Triton X-100 extracts of basal forebrain and cerebellum by incubating them with Sepharose 4B covalently bound with α-Bgtx. The bound receptors were eluted using sample buffer and an identical volume of purified receptors was loaded on the gel. The Western blots were probed with anti-α₇ Ab (top) or anti-β₂ Ab (bottom).