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Isometric myography. SKA-111 and SKA-121 (both at 1 µM) increased BK-induced EDH-type relaxation of U46619-precontracted PCA rings in the presence of blockers of NO synthesis (l-NNA) and cyclooxygenases (indomethacin). TRAM-34 (1 µM) alone and the combination of TRAM-34 and UCL-1684 (1 µM) prevented the increase of relaxation. Data are mean ± S.E.M., n = 5–22 PCA. *P < 0.05, unpaired Student’s t test.
