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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Am J Respir Crit Care Med. 2004 Oct 29;171(3):204–211. doi: 10.1164/rccm.200403-313OC

Figure 2. Distribution of gene delivery and expression in electroporated rat lungs.

Figure 2

A and B. GFP-β1 expressing plasmids (600 μg) were administered to the lungs and electroporated (200 V/cm, 8 pulses at 10 μsec each). Three days later, the lungs were visualized in situ (A) and GFP-β1 expression was detected by fluorescence microscopy (B). C and D. Dose-dependency of gene transfer and expression. Two hundred micrograms (C) or 600 μg (D) of a β-galactosidase-expressing plasmid was transferred to lungs and electroporated (200 V/cm, 8 pulses at 10 μsec each). Three days later, the lungs were removed and reacted with X-gal to visualize β-galactosidase expression.