(A) U2OS cells at different cell cycle stages were stained with indicated antibodies. DAPI (blue) marks nuclei.
(B) CEP295 localizes to the extra daughter centrioles of the rosette centrosome, inducibly formed by Plk4 overexpression in RPE1 cells.
(C) G1, or S/G2 centrioles were visualized with indicated antibodies, including the proximal end marker C-Nap1 or SAS-6, and distal end marker centrin or Odf2.
(D) CEP295 signals were lost in CEP295-RNAi cells, revealed by both immunostaining and western blot.