Table 1.
Antigen | Code | Species | Dilution | Supplier |
---|---|---|---|---|
Primary antibodies | ||||
Adenosine receptor A1 | AB1587P | Rabbit | 1 : 50 | Chemicon |
Adenosine receptor A2A | A2aR21-A | Rabbit | 1 : 150 | Alpha Diagnostics |
NTPDase1 | rN1-6LI4 | Rabbit | 1 : 1000 | http://ectonucleotidases-ab.com/ |
NTPDase2 | rN2-6L | Rabbit | 1 : 400 | http://ectonucleotidases-ab.com/ |
NTPDase3 |
rN3-1LI5 | Rabbit | 1 : 150 | http://ectonucleotidases-ab.com/ |
NTPDase8 |
rN8-8CI5 | Guinea-pig | 1 : 600 | http://ectonucleotidases-ab.com/ |
Ecto-5′-nucleotidase |
rNu-9LI5 | Rabbit | 1 : 1000 | http://ectonucleotidases-ab.com/ |
ADA | sc-25747 | Rabbit | 1 : 50 | Santa Cruz |
Secondary antibodies | ||||
Alexa Fluor 488 anti-rb | A-21206 | Donkey | 1 : 1000 | Molecular probes |
TRITC 568 anti-gp | 706-025-148 | Donkey | 1 : 150 | Molecular probes |