Figure 6.

Effect of α-LA on activation of β-catenin in H. pylori-infected AGS cells. AGS cells were pretreated with α-LA for 2 h and cultured in the presence or absence of H. pylori for 24 h. (a) Protein levels of β-catenin in cytosolic and nuclear extracts were determined by Western blot analysis. Aldolase A and histone H1 were used as indices for cytosolic and nuclear extracts, respectively. (b) Immunofluorescence staining was performed to determine the levels of β-catenin in nucleus and cytosol. β-catenin was visualized using fluorescein isothiocyanate-conjugated anti-mouse IgG antibody (lower panel) with DAPI counter staining (upper panel) of the same field.