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CM from HMEC in simulated microgravity inhibit NHOst activity. NHOst were cultured for 7 and 14 days with medium conditioned by HMEC in simulated microgravity (CM-RWV) or by HMEC controls (CM-C) both added with osteogenic stimuli. (a) ALP enzymatic activity was quantified by spectrophotometric analysis as described. Absorbance was measured at 405 nm. (b) Alizarin Red Staining was performed. Photographs were taken before acid extraction. Absorbance was measured at 562 nm.
