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CM from HMEC in simulated microgravity inhibit Saos-2 proliferation. Saos-2 were cultured for different times with CM from HMEC in simulated microgravity (CM-RWV) or by HMEC controls (CM-C). Viable cells were evaluated by MTT assay (a) and the absolute absorbance values are shown. After trypsinization, viable cells were stained with trypan blue and counted (b).
