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. 2014 Sep 3;5:437. doi: 10.3389/fpls.2014.00437

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Copyright © 2014 Herman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A comparison of the expression of the storage protein trait in somatic embryos and seeds is shown as a GFP-HDEL glycinin mimic allele expressed as a storage protein proxy. The somatic embryo in white light (A) and UV fluorescence (B) shows that the primary GFP expression site is in the cotyledon that is reduced in size compared to cotyledons of seed. In seeds the cotyledons comprise the large majority of the seed’s mass shown as a chipped seed illuminated with white light (C) and UV for GFP fluorescence (D). The comparative expression the same glycinin promoter-regulated GFP in somatic embryos and seeds shows that while somatic embryos are a good proxy for seed expression there are challenges in interpreting somatic embryo results as an accurate proxy for a seed’s in planta protein content and composition.