Table 2.
Annotated secreted Botrytis enzymes involved in plant cell wall disassembly.
| Plant cell wall target | CAZyme subfamily | Functional annotation | Proteins potentially secreted | Genes expressed in all three hosts |
|---|---|---|---|---|
| HG backbone | GH28 | Polygalacturonases | 11 | BcPG1, BcPG3, BcPG4, BcPG5, BcPG6, BC1G_00240, BC1G_01617, BC1G_11909, BC1G_15118 |
| PL1 | Pectin lyases | 4 | BC1G_07527, BC1G_11690, BC1G_12017, BC1G_12517 | |
| PL1, PL3 | Pectate lyases | 4 | BC1G_00912, BC1G_09000, BC1G_07052, BC1G_10229 | |
| CE8 | Pectin methylesterases | 3 | BcPME1, BcPME2, BC1G_11144 | |
| RG-I backbone | GH28, GH105|GH28 | RG hydrolases | 6 | BC1G_01234, BC1G_01923, BC1G_03619, BC1G_03464, BC1G_05961, BC1G_13970 |
| GH78 | α-L-Rhamnosidases | 2 | BC1G_06328 | |
| CE12 | RG acetylesterases | 1 | BC1G_14009 | |
| XyG backbone | GH3 | β-Glucosidases | 6 | BC1G_03179, BC1G_07110, BC1G_07622, BC1G_10231, BC1G_11439, BC1G_14169 |
| GH12 | XyG-specific β-glucanases | 1 | BC1G_00594 | |
| GH16, GH16|CBM18 | Glucanases and XyG transglucosylase/hydrolases | 11 | BC1G_00409, BC1G_02932, BC1G_04948, BC1G_07945, BC1G_08924, BC1G_09991 | |
| Xylan backbone | GH10, GH11, GH10|CBM1, GH11|CBM1 | β-Xylanases | 5 | BcXyn11A, BC1G_00576, BC1G_01778, BC1G_03590 |
| GH43 | β-Xylosidases | 3 | BC1G_02487, BC1G_10797 | |
| Mannans | GH5|CBM1 | β-Mannosidases | 1 | BC1G_02036 |
| GH26, CBM3|GH26| CBM35|GH44 | β-Mannanases | 2 | BC1G_10341 | |
| Cellulose | GH5, GH5|CBM1, GH45 | 1,4-β-Glucanases | 10 | BC1G_02740, BC1G_03038, BC1G_07822, BC1G_08990, BC1G_09210, BC1G_13855, BC1G_13862, BC1G_16238, BcCEL5A, |
| GH6|CBM1, GH6|CBM2, GH7, GH7|CBM1 | Cellulose 1,4-β-cellobiosidases | 5 | BC1G_06035, BC1G_08989, BC1G_10880, BC1G_13445, BC1G_14702 | |
| Side-chains/adducts | GH2, GH35 | β-Galactosidases | 3 | BC1G_02410, BC1G_03567, BC1G_12184 |
| GH31 | α-Xylosidases | 2 | BC1G_12859, BC1G_11115 | |
| GH43, GH93 | α-L-1,5-Arabinanases | 2 | BcAra1, BC1G_13938 | |
| GH47, GH92 | α-Mannosidases | 4 | BC1G_00245, BC1G_00455, BC1G_09742, BC1G_12174 | |
| GH51, GH54|CBM42, GH62|CBM13 | α-Arabinofuranosidases | 4 | BC1G_04994, BC1G_08372, BC1G_10789, BC1G_12138 | |
| GH53 | AG β-galactosidases | 1 | BC1G_16209 | |
| GH95 | α-L-Fucosidases | 1 | BC1G_08975 | |
| GH115 | Xylan α-1,2-glucuronosidases | 1 | BC1G_13153 |
The secreted CAZyme-coding genes that were commonly expressed during Botrytis infections of lettuce leaves, tomato fruit and grape berries are presented.