Table 5.
Gene expression analysis by real-time PCR in brown adipose tissue after administration of asperuloside (ASP) under high-fat diet (HFD) conditions
(Mean values with their standard errors)
| HFD-ASP (fold of Cont.) | HFD-ELE (fold of Cont.) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| HFD-Cont. (n 6) | 0·03 % (n 6) | 0·1 % (n 6) | 0·3 % (n 6) | 5 % (n 6) | ||||||
| Gene name | Mean | se | Mean | se | Mean | se | Mean | se | Mean | se |
| Uncoupling ATP synthesis from oxidative metabolism | ||||||||||
| UCP1* | 1·00 | 0·07 | 0·98 | 0·04 | 1·38 | 0·10 | 1·98 | 0·07 | 1·01 | 0·05 |
| UCP2*† | 1·00 | 0·08 | 1·10 | 0·07 | 1·42 | 0·13 | 1·64 | 0·07 | 2·26 | 0·14 |
| UCP3 | 1·00 | 0·07 | 0·96 | 0·06 | 0·92 | 0·06 | 0·89 | 0·04 | 1·09 | 0·02 |
Cont., Control; ELE, Eucommia leaf extract; UCP, uncoupling protein.
* Linear contrast with Control (0 %), 0·03 %, 0·1 % and 0·3 % ASP (P < 0·05).
† Contrast for Control v. 5 % ELE (P < 0.05).