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. 2014 Sep 3;8:86. doi: 10.3389/fnana.2014.00086

Figure 2.

Figure 2

Shuttle vector and landing pad system for streamlining IE180-null PRV mutant recombinants. (A) A landing pad was inserted into the PRV IE180-null BAC. The transgene is inserted into a shuttle plasmid containing homology arms (h.a.) to the landing pad. The transgene is then amplified together with the Zeocin selection cassette using standardized primers and inserted into the IE180-null BAC using recombineering. (B–D) PK15 cells infected with PRV strains expressing EGFP (PRV HKO128), mCherry (PRV HKO242) and ECFP (PRV HKO243). (E) Dissociated neurons infected with PRV HKO128, at 3 weeks post infection.