Fig. 4.
Effects of repetitive PCI PrS-CD gene transfection. F98 or U87 spheroids were transfected with 1 or 2 complete PCI cycles. 50 μM of 5-FC was added after light treatment per session. Spheroid growth was observed for a maximum period of 21 days. a Low power micrograph after 14 days for F98 cell spheroids. b growth kinetics of CD transfected F98 and U87 spheroids following single (91) or double treatment (×2). CD 2 and 4 in the figure refers to μg/ml of CD DNA plasmid used. The results are shown as a percentage of PDT controls ± SE. Each bar represents the average of 24 replicate cultures from 3 independent experiments