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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Dev Cell. 2011 Aug 16;21(2):245–256. doi: 10.1016/j.devcel.2011.06.026

Figure 1. iGFPi Is an Effective Method to Knock Down the Expression of GFP-Trapped Proteins.

Figure 1

(A) Strategy of in vivo GFP interference (iGFPi). Knockdown of GFP-trapped proteins is achieved through RNAi targeting the GFP-encoding sequence.

(B) Expression of nub-Gal4 in the blade region of a third instar (L3) wing disc, revealed by Gal4-dependent expression of RFP (UAS-myrRFP, red). Cell nuclei stained with DAPI (blue) on left.

(C) Wild-type adult wing.

(D and E) iGFPi driven by nub-Gal4 (nub>iGFPi) reduces expression of GFP-trapped Strawberry notch (Sno) (E, compared to control in D; GFP in green) and causes delta-shaped termination of veins at the margin (E’).

(F and G) nub>iGFPi reduces expression of GFP-trapped Scalloped (Sd) and causes loss of distal wing tissue (G’).

(H and I) nub>iGFPi reduces expression of GFP-trapped Neuroglian (Nrg) and causes reduction in wing size (I’).

(J and K) nub>iGFPi reduces expression of GFP-trapped Discs large (Dlg) and prevents correct differentiation (K’).

(L and M) iGFPi driven by He-Gal4 in the salivary gland reduces expression of GFP-trapped Bunched (Bun) and causes reduction in cell and organ size.

(N and O) iGFPi driven by ey-Gal4 in the eye of flies expressing GFP-trapped Discs large (Dlg) prevents correct differentiation.

See also Figure S1.