a, DC subsets were isolated from PBS-perfused lung tissue for microarray analysis. Bars show relative mRNA expression of the indicated genes in pulmonary CD103+ DCs and CD11b+ DCs treated +/- PIC or R848. b, FACS analysis of migratory DCs isolated from LLN of IL-12p40 reporter mice, 24h post i. n. instillation of 10 μg Poly I:C (PIC) or 50 μg R848. c,d, OT-I CD8 T cells were adoptively transferred into WT, IL-12-/-, IL-27-/-, or IL-12-/-IL-27-/- mice 1d prior to i.n. delivery of PBS, soluble OVA or OVA+ apoptotic cells +/- 10 μg Poly I:C (PIC) or 50 μg R848. Five days after immunization, mice were given PBSE-labeled target (OVA— or OVA+) cells. 24h later spleens were assessed for target cell frequency. Each dot represents one mouse. Data represents at least 3 independent experiments with 3-4 mice/group. **p< 0.0015, *p<0.015, t-test..