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. 2014 May 23;7(9):1441–1454. doi: 10.1093/mp/ssu065

Figure 1.

Figure 1

Inhibition of Proteolysis Impairs ABA-Induced Stomatal Closure and Decreases in GFP-Tubulin Fluorescence.

(A) Stomatal apertures (n > 50) are plotted from leaf peels either pre-treated with DMSO or (DMSO + 50 μM MG-132) for 1 h, followed by 10 μM ABA treatment as indicated.

(B) Relative changes in total GFP-tubulin fluorescence in guard cells is plotted over time during the ABA treatment. Inhibition of proteolysis attenuated the loss of total fluorescence in response to ABA treatment.

(C) Confocal images of GFP:tubulin-labeled guard cells either pre-treated with DMSO, or DMSO + MG-132. Data shown are from one of three independent repeats, each with similar results. Images were taken as described in the ‘Methods’ section. Error bars show ±SE. Scale bar = 5 μm.