Figure 1.

opt3-2 Over-Accumulates Cd in Seeds and Is Cd-Hypersensitive.

(A) Ionomic profile of opt3-2 seeds grown on soil supplemented with heavy metals. Metal concentrations were determined by ICP–OES, normalized against Mg, and plotted as standard deviation from the wild-type mean (Z-value) (Lahner et al., 2003). Each line represents seeds from independent plants grown on heavy metal-laden soil. Z-values are considered significant when |z| > 1.96 (p < 0.05). (B) opt3-2 seedlings are hypersensitive to Cd. Wild-type and opt3-2 seeds were grown on ¼ MS media with or without 50 μM CdCl₂ for 2 weeks_.