Figure 4.

Ectopic Overexpression of OPT3 in opt3-2 Reduces Cadmium Concentration in Seeds and Rescues the Seedling Hypersensitivity to Cd.

(A) Relative OPT3 expression levels of four representative 35S _pro:OPT3 overexpression lines. Wild-type, opt3-2, and four OPT3 overexpression lines were grown on ¼ MS for 2 weeks, and OPT3 expression was determined by qPCR and normalized against wild-type OPT3 expression levels. Data represent mean ± SE (n = 3).

(B) OPT3 overexpression reduces the over-accumulation of Cd in seeds and (C) the Cd hypersensitivity of opt3-2 seedlings. Wild-type, opt3-2, and four complemented lines were grown on ¼ MS with or without 50 μM CdCl₂ for 2 weeks. Data represent mean ± SE (n = 6; * p < 0.05).