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. 2014 May 31;7(9):1455–1469. doi: 10.1093/mp/ssu067

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© The Author 2014. Published by Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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OPT3 Is a Plasma Membrane Transporter Expressed in the Phloem.

(A, B) GUS staining was performed in fixed and sectioned stems of OPT3 _pro:GUS plants under (A) iron-deficient conditions and (B) Fe-sufficient conditions.

(C) OPT3 localizes to the plasma membrane. N. benthamiana epidermal cells were infiltrated with Agrobacterium carrying 35S _pro:YFP–OPT3 and imaged using confocal microscopy 3 d later. Fluorescence in the cell perimeter is indicative of plasma membrane localization. ER fluorescence is also present (arrows) surrounding the nucleus and as strands traversing the cytoplasm.

(D) Leaves of N. benthamiana epidermal cells transiently expressing YFP–OPT3 as in the panel were plasmolyzed with 4% NaCl. Note the Hechtian strands (arrows) connecting the cell wall to the plasmolyzed protoplast (double arrowheads), indicative of plasma membrane localization.