Table 2.
RNA in situ hybridization and antibody probes
| RNA/antibody | Properties | Source |
|---|---|---|
|
islet1 |
1,900 bp |
Dr Judith Eisen, Institute of Neuroscience, University of Oregon, Eugene, OR, USA |
|
nrp1a |
1,200 bp |
Dr Waturu Shoji, Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan |
|
vglut2.1 |
500 bp |
Dr Shin-ichi Higashijima, Okazaki Institute of Integrative Bioscience, Okazaki, Japan |
|
vglut2.2 |
500 bp |
|
|
gad65 |
900 bp |
|
|
gad67 |
900 bp |
|
|
olig4 |
1000 bp |
Dr Francesco Argenton, Department of Biology, University of Padua, Padua, Italy |
|
scn8aa |
655 bp |
Our work |
|
runx3 |
1,900 bp |
Dr Phil Crosier, Dept Mol Medicine, University of Auckland, Auckland, New Zealand |
| anti-GFP |
Rabbit polyclonal, (1:700) |
Molecular Probes-Life Technologies, Grand Island, NY, USA |
| anti-zn12 (HNK) |
Mouse monoclonal, (1:100) |
DSHB* |
| anti-GABA |
Rabbit polyclonal, (1:250) |
Sigma-Aldrich Co., St. Louis, MO, USA |
| goat anti-rabbit |
Conjugated to Alexa-568/488, (1:1000) |
Molecular Probes-Life Technologies |
| goat anti-mouse | Conjugated to Alexa-488, (1:1000) | Molecular Probes-Life Technologies |
*DSHB, Developmental Studies Hybridoma Bank; the zn12 monoclonal antibody was developed under the auspices of the National Institute of Child Health and Development and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA, USA. bp, Base pair; GABA, γ-aminobutyric acid; GFP, green fluorescent protein.