Table 3. Wolbachia MLST Results.
loci | California | Florida | bp / aa differences between CA and FL |
gatB | 9 | 227* | 3 |
coxA | 212* | 221* | 14 |
hcpA | 88 | 251* | 1 |
ftsZ | 195* | 195* | 0 |
fbpA | 27 | 403* | 2 |
wsp | 378 | 675* | 59a |
HVR1 | 150 | 233* | 4 |
HVR2 | 165 | 266* | 10b |
HVR3 | 24 | 8 | 7 |
HVR4 | 3 | 7 | 10 |
59 differences include 56 substitutions and 3 indels (3 bp each).
10 differences include 8 substitutions and 2 indels (1 aa each).
Five individual cloned PCR products were sequenced for each locus from each insect population. Sequences from all three California populations (Bakersfield, Ojai, and colony) were identical and have been grouped together. Allele numbers are from http://pubmlst.org/wolbachia/ and novel alleles are marked (*). Differences are base pairs for genes (gatB, coxA, hcpA, ftsZ, fbpA, and wsp) and amino acids for peptides (HVR1, 2, 3 and 4).