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. 2014 Sep 3;9(9):e106215. doi: 10.1371/journal.pone.0106215

Table 5. Primers used to amplify 16S DNA.

Forward primers Reverse primers
NNNNNNAAACTCAAAGGAATTGACGG NNNNNNGGGTTGCGCTCGTTGCGG
NNNNNNAACTCAAAGGAATTGACGGG NNNNNNGGTTGCGCTCGTTGCGGG
NNNNNNACTCAAAGGAATTGACGGGG NNNNNNGTTGCGCTCGTTGCGGGA
NNNNNNCTCAAAGGAATTGACGGGGR NNNNNNTTGCGCTCGTTGCGGGAC
NNNNNNTCAAAGGAATTGACGGGGRC NNNNNNTGCGCTCGTTGCGGGACT
NNNNNNCAAAGGAATTGACGGGGRCC NNNNNNGCGCTCGTTGCGGGACTT

Equimolar amounts of all 6 forward primers and all 6 reverse primers were pooled and used in PCR. Forward primers span nt 926–950; reverse primers span nt 1107–1129 (numbering relative to E. coli 16S DNA).