Table 2. Phenotypic characterization of 763 strains representing the genetic diversity in the P. syringae complex.
Phylogenetic affiliation | Number of strains | Levan + | Oxidase + | Potato soft rot | HR on tobacco+ | Fluorescence on KB medium + | Aesculin degradation + | Sucrose utilization+ | D(-) Tartrate utilization + | I NAb + | Broad host-range toxinc + | Pathogenicityd+ | Avirulente | Mean sdisease severity ≥2.0f |
Total | 763 | 41 | 0 | 10 | 73 | 98 | 97 | 73 | 32 | 65 | 51 | 28 | 53 | 12 |
PG a 01 | 88 | 39 | 0 | 0 | 92 | 85 | 92 | 91 | 58 | 33 | 2 | 16 | 60 | 1 |
PG 01a | 48 | 45 | 0 | 0 | 90 | 100 | 100 | 88 | 78 | 24 | 4 | 14 | 53 | 2 |
PG 01b | 40 | 33 | 0 | 0 | 95 | 68 | 83 | 95 | 35 | 43 | 0 | 18 | 68 | 0 |
PG 02 | 323 | 50 | 0 | 0 | 72 | 100 | 100 | 95 | 10 | 85 | 90 | 43 | 41 | 25 |
PG 02a | 3 | 0 | 0 | 0 | 100 | 100 | 100 | 100 | 33 | 67 | 67 | 33 | 33 | 0 |
PG 02b | 140 | 67 | 0 | 0 | 98 | 100 | 99 | 95 | 4 | 90 | 87 | 44 | 30 | 19 |
PG 02c | 91 | 7 | 0 | 0 | 7 | 100 | 100 | 93 | 9 | 68 | 96 | 0 | 89 | 0 |
PG 02d | 87 | 70 | 0 | 0 | 100 | 100 | 100 | 98 | 20 | 93 | 91 | 89 | 6 | 62 |
PG 02e | 2 | 0 | 0 | 0 | 50 | 100 | 100 | 100 | 0 | 100 | 100 | 0 | 100 | 0 |
PG 03 | 10 | 70 | 0 | 0 | 80 | 70 | 20 | 90 | 0 | 20 | 0 | 0 | 90 | 0 |
PG 04 | 6 | 83 | 0 | 0 | 100 | 100 | 100 | 100 | 0 | 100 | 17 | 0 | 67 | 0 |
PG 05 | 4 | 50 | 0 | 0 | 75 | 75 | 25 | 75 | 25 | 50 | 0 | 25 | 50 | 0 |
PG 07 | 81 | 46 | 0 | 98 | 62 | 100 | 100 | 9 | 96 | 33 | 0 | 47 | 37 | 11 |
PG 07a | 79 | 46 | 0 | 97 | 61 | 100 | 100 | 9 | 96 | 33 | 0 | 48 | 37 | 11 |
PG 07b | 2 | 50 | 0 | 100 | 100 | 100 | 100 | 0 | 100 | 50 | 0 | 0 | 50 | 0 |
PG 08 | 5 | 80 | 0 | 40 | 100 | 100 | 80 | 0 | 100 | 0 | 100 | 0 | 80 | 0 |
PG 09 | 28 | 11 | 0 | 0 | 68 | 100 | 100 | 14 | 32 | 4 | 0 | 0 | 71 | 0 |
PG 09a | 23 | 9 | 0 | 0 | 65 | 100 | 100 | 13 | 26 | 4 | 0 | 0 | 70 | 0 |
PG 09b | 4 | 25 | 0 | 0 | 75 | 100 | 100 | 25 | 50 | 0 | 0 | 0 | 75 | 0 |
PG 09c | 1 | 0 | 0 | 0 | 100 | 100 | 100 | 0 | 100 | 0 | 0 | 0 | 100 | 0 |
PG 10 | 167 | 34 | 0 | 0 | 98 | 100 | 100 | 84 | 11 | 94 | 51 | 10 | 66 | 3 |
PG 10a | 71 | 8 | 0 | 0 | 97 | 100 | 100 | 82 | 23 | 94 | 1 | 7 | 80 | 1 |
PG 10b | 77 | 58 | 0 | 0 | 100 | 100 | 100 | 86 | 3 | 94 | 91 | 1 | 62 | 0 |
PG 10c | 1 | 0 | 0 | 0 | 100 | 100 | 100 | 0 | 0 | 100 | 0 | 0 | 100 | 0 |
PG 10d | 8 | 13 | 0 | 0 | 100 | 100 | 100 | 75 | 0 | 100 | 75 | 50 | 25 | 13 |
PG 10e | 8 | 50 | 0 | 0 | 88 | 100 | 100 | 100 | 0 | 100 | 100 | 75 | 13 | 38 |
PG 10f | 1 | 100 | 0 | 0 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | 100 | 0 |
PG 10g | 1 | 0 | 0 | 0 | 0 | 100 | 100 | 100 | 0 | 0 | 0 | 0 | 100 | 0 |
PG 11 | 3 | 0 | 100 | 0 | 67 | 100 | 100 | 0 | 33 | 0 | 100 | 67 | 33 | 0 |
PG 12 | 2 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 50 | 0 | 0 | 0 | 100 | 0 |
PG 12a | 1 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
PG 12b | 1 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 100 | 0 | 0 | 0 | 100 | 0 |
PG 13 | 46 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 100 | 0 | 0 | 0 | 78 | 0 |
PG 13a | 44 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 100 | 0 | 0 | 0 | 77 | 0 |
PG 13b | 2 | 0 | 0 | 0 | 0 | 100 | 100 | 0 | 100 | 0 | 0 | 0 | 100 | 0 |
Values are the percent of P. syringae strains giving positive reactions for the different phenotypes. Arginine dihydrolase production was negative for all strains.
PG = P. syringae phylogroup.
INA = ice nucleation activity of at least 106 cells at >−8°C.
Production of a broad host range toxin was evaluated with the test habitually used to reveal syringomycin-like toxins based on the capacity to produce an inhibition zone of growth of Geotricum candidum.
Strains were considered to be pathogenic on the cantaloupe indicator plant if at least half (6/12) of the seedlings showed compatible reactions.
Avirulent strains did not induce any disease reaction on cantaloupe seedlings.
Frequency of strains for which the mean disease severity on cantaloupe seedlings was ≥2.0.