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. 2014 Sep 3;9(9):e105547. doi: 10.1371/journal.pone.0105547

Table 2. Phenotypic characterization of 763 strains representing the genetic diversity in the P. syringae complex.

Phylogenetic affiliation Number of strains Levan + Oxidase + Potato soft rot HR on tobacco+ Fluorescence on KB medium + Aesculin degradation + Sucrose utilization+ D(-) Tartrate utilization + I NAb + Broad host-range toxinc + Pathogenicityd+ Avirulente Mean sdisease severity ≥2.0f
Total 763 41 0 10 73 98 97 73 32 65 51 28 53 12
PG a 01 88 39 0 0 92 85 92 91 58 33 2 16 60 1
PG 01a 48 45 0 0 90 100 100 88 78 24 4 14 53 2
PG 01b 40 33 0 0 95 68 83 95 35 43 0 18 68 0
PG 02 323 50 0 0 72 100 100 95 10 85 90 43 41 25
PG 02a 3 0 0 0 100 100 100 100 33 67 67 33 33 0
PG 02b 140 67 0 0 98 100 99 95 4 90 87 44 30 19
PG 02c 91 7 0 0 7 100 100 93 9 68 96 0 89 0
PG 02d 87 70 0 0 100 100 100 98 20 93 91 89 6 62
PG 02e 2 0 0 0 50 100 100 100 0 100 100 0 100 0
PG 03 10 70 0 0 80 70 20 90 0 20 0 0 90 0
PG 04 6 83 0 0 100 100 100 100 0 100 17 0 67 0
PG 05 4 50 0 0 75 75 25 75 25 50 0 25 50 0
PG 07 81 46 0 98 62 100 100 9 96 33 0 47 37 11
PG 07a 79 46 0 97 61 100 100 9 96 33 0 48 37 11
PG 07b 2 50 0 100 100 100 100 0 100 50 0 0 50 0
PG 08 5 80 0 40 100 100 80 0 100 0 100 0 80 0
PG 09 28 11 0 0 68 100 100 14 32 4 0 0 71 0
PG 09a 23 9 0 0 65 100 100 13 26 4 0 0 70 0
PG 09b 4 25 0 0 75 100 100 25 50 0 0 0 75 0
PG 09c 1 0 0 0 100 100 100 0 100 0 0 0 100 0
PG 10 167 34 0 0 98 100 100 84 11 94 51 10 66 3
PG 10a 71 8 0 0 97 100 100 82 23 94 1 7 80 1
PG 10b 77 58 0 0 100 100 100 86 3 94 91 1 62 0
PG 10c 1 0 0 0 100 100 100 0 0 100 0 0 100 0
PG 10d 8 13 0 0 100 100 100 75 0 100 75 50 25 13
PG 10e 8 50 0 0 88 100 100 100 0 100 100 75 13 38
PG 10f 1 100 0 0 100 100 100 100 100 100 100 0 100 0
PG 10g 1 0 0 0 0 100 100 100 0 0 0 0 100 0
PG 11 3 0 100 0 67 100 100 0 33 0 100 67 33 0
PG 12 2 0 0 0 0 100 100 0 50 0 0 0 100 0
PG 12a 1 0 0 0 0 100 100 0 0 0 0 0 100 0
PG 12b 1 0 0 0 0 100 100 0 100 0 0 0 100 0
PG 13 46 0 0 0 0 100 100 0 100 0 0 0 78 0
PG 13a 44 0 0 0 0 100 100 0 100 0 0 0 77 0
PG 13b 2 0 0 0 0 100 100 0 100 0 0 0 100 0

Values are the percent of P. syringae strains giving positive reactions for the different phenotypes. Arginine dihydrolase production was negative for all strains.

a

PG =  P. syringae phylogroup.

b

INA = ice nucleation activity of at least 106 cells at >−8°C.

c

Production of a broad host range toxin was evaluated with the test habitually used to reveal syringomycin-like toxins based on the capacity to produce an inhibition zone of growth of Geotricum candidum.

d

Strains were considered to be pathogenic on the cantaloupe indicator plant if at least half (6/12) of the seedlings showed compatible reactions.

e

Avirulent strains did not induce any disease reaction on cantaloupe seedlings.

f

Frequency of strains for which the mean disease severity on cantaloupe seedlings was ≥2.0.