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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Addict Biol. 2011 Aug 4;17(2):351–364. doi: 10.1111/j.1369-1600.2011.00357.x

Figure 3.

Figure 3

Confirmation of select genes of interest by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in the (a) prefrontal cortex and (b) hippocampus of chronic intermittent ethanol exposure (CIE) and ethanol early withdrawal (EWD)-treated mice. The complete primer sequences for the gene selected are listed in Supporting Information Table S1. Total RNA isolated for microarray hybridizations was used for mRNA expression by qRT-PCR. Gene and mRNA expression values were normalized as percent of controls. For most of the genes tested, changes in gene expression identified with microarray analyses for CIE and EWD conditions were confirmed by qRT-PCR analyses. Significance was determined by one-way analysis of variance (*P < 0.05; n = 4/group)