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. 2014 Sep 3;9(9):e106482. doi: 10.1371/journal.pone.0106482

Figure 5. Effects of temperature and pH on the activity and stability of Xyn10A and Agu67A.

Figure 5

A. Temperature profile of Xyn10A. Xylanase activity determination was performed in a temperature range of 40–95°C at pH 6.0 for 3 min. B. pH profile of Xyn10A. Xylanase activity assay was carried out by a 3 min incubation using phosphate-citrate buffers (pH 4.0–8.5) at 80°C. C. Thermostability profile of Xyn10A. The purified Xyn10A was incubated in pH 8.5 buffer at 75, 80 and 85°C, respectively for 0.5, 1, 2, 3, 4 and 6 h, and residual activity was detected under optimal conditions. D. pH stability profile of Xyn10A. The purified Xyn10A was pre-incubated in pH 4.0–8.5 buffers at room temperature for 10 h, and then the residual activity was measured under optimal conditions. E. Temperature profile of Agu67A. The α-glucuronidase activity determination was performed in a temperature range of 40-95°C at pH 6.5 for 5 min. F. pH profile of Agu67A. The α-glucuronidase activity assay was carried out by a 5 min incubation using phosphate-citrate buffers (pH 4.0–8.5) at 75°C. The maximum activity was defined as 100% and values shown were the means of three replicates.