Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 3;9(9):e106482. doi: 10.1371/journal.pone.0106482

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Jia et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. TLC analysis of the XOs hydrolysis products. The detectable xylose produced by Agu67A was marked with a box. B. Produced reducing sugar assay of the XOs hydrolysis products. C. TLC analysis of the beechwood xylan hydrolysis products after different incubation times. D. Produced reducing sugar assay of the beechwood xylan hydrolysis products. The differences of hydrolysis products after 1 hour were marked with a box. E. HPLC analysis of the beechwood xylan hydrolysis products of 2 hours. F. Details of the HPLC analysis (retention time 13.5–14.5 min). XOs hydrolysis was performed by incubating single or mixed enzyme (2.0 µM each, final concentration) with 2.0% (w/v) XOs at 80°C and pH 6.5 for 4 hours. Beechwood xylan hydrolysis was conducted by incubating Xyn10A (1.75 µM, final concentration), or Agu67A (0.85 µM, final concentration), or Xyn10A (1.75 µM, final concentration) and Agu67A (0.85 µM, final concentration) mixture with 1.0% (w/v) beechwood xylan at 80°C and pH 6.5 for different times (0, 0.25, 1, 2, 6, 12 hours). Xylose (X₁), xylobiose (X₂), xylotriose (X₃), and xylotetraose (X₄) were used as standards and labeled.