Table 1. Final screening results of hybridoma cells resulted from fusions of splenocytes.
Clone | Titera of supernatants | OD 450 nm values | Blank | |
STGb | ||||
100 ng/mL | 0 ng/mL | |||
VerA 3 | 800 | 0.1012 | 1.053 | 0.0873 |
VerA 4 | 400 | 0.1123 | 1.032 | 0.0972 |
VerA 6 | 50 | 0.1240 | 0.986 | 0.0108 |
The titer was defined as the reciprocal of the dilution that gave an absorbance most close to 1.0.
STG was prepared by diluting the stock solution with 10% methanol-PBS to 200 ng mL−1 and mixed with equivalent supernatants, and the final concentrations were 100 ng mL−1. Aflatoxins of 0 ng mL−1 were the PBS containing the same methanol content.