Table 2. Titers and isotypes of ascites antibodies.
Clone | Titera | OD 450 nm | Value | Isotype | ||
Negative control serum | 1% BSA | Blank | Heavychain | Lightchain | ||
VerA 3 | 6.4×105 | 0.093 | 0.091 | 0.088 | IgG1 | Kappa |
VerA 4 | 3.2×105 | 0.106 | 0.102 | 0.097 | IgG2a | Kappa |
VerA 6 | 3.2×105 | 0.097 | 0.089 | 0.095 | IgG2a | Kappa |
The titer was defined as the reciprocal of the dilution that gave an absorbance most close to 1.0.