Table 4. Top 10 small RNA reads of M. smegmatis.
ID | % of RISC-associated miRNA reads | 5′ position in genome | Sense | Length (nt) | Origin | Stem-loop structure prediction | RIP-enrichment (fold) |
MS-A | 2.40 | 3325937 | - | 16 | ORF (as) | No | 25.8 |
MS-B | 0.62 | 658512 | - | 16 | ORF (as) | No | 27.2 |
MS-C | 0.61 | 5091600 | + | 15 | ORF | Noncanonical a | 27.0 |
MS-D | 0.27 | 1454138 | - | 16 | ORF (as) | Noncanonical | 25.8 |
MS-E | 0.24 | 3325937 | - | 17 | ORF (as) | No | 15.5 |
MS-F | 0.16 | 4060958 | - | 16 | ORF (as) | Noncanonical | 40.8 |
MS-G | 0.09 | 4987681 | - | 16 | ORF | Noncanonical | 174.5 |
MS-H | 0.08 | 6366753 | - | 16 | ORF (as) | Noncanonical | 43.3 |
MS-I | 0.08 | 4998098 | - | 18 | ORF | Noncanonical | 56.0 |
MS-J | 0.07 | 4048594 | + | 17 | pseudo gene (as) | Noncanonical | 132.3 |
The predicted stem-loop structures and origin of these small RNAs is depicted in Fig. S4.