Table 4. Top 10 small RNA reads of M. smegmatis.

ID	% of RISC-associated miRNA reads	5′ position in genome	Sense	Length (nt)	Origin	Stem-loop structure prediction	RIP-enrichment (fold)
MS-A	2.40	3325937	-	16	ORF (as)	No	25.8
MS-B	0.62	658512	-	16	ORF (as)	No	27.2
MS-C	0.61	5091600	+	15	ORF	Noncanonical a	27.0
MS-D	0.27	1454138	-	16	ORF (as)	Noncanonical	25.8
MS-E	0.24	3325937	-	17	ORF (as)	No	15.5
MS-F	0.16	4060958	-	16	ORF (as)	Noncanonical	40.8
MS-G	0.09	4987681	-	16	ORF	Noncanonical	174.5
MS-H	0.08	6366753	-	16	ORF (as)	Noncanonical	43.3
MS-I	0.08	4998098	-	18	ORF	Noncanonical	56.0
MS-J	0.07	4048594	+	17	pseudo gene (as)	Noncanonical	132.3

^aThe predicted stem-loop structures and origin of these small RNAs is depicted in Fig. S4.