Table 1. Primers for site-directed mutagenesis.

Primer	Sequence (from 5′ to 3′ end)
R105A	Fwd: CTGTCTTCACAAACGCGAGGTCTTTTGGTCC
	Rev: GGACCAAAAGACCTCGCGTTTGTGAAGACAG
R106A	Fwd: TATTCTGTCTTCACAAACCGGGCGTCTTTTGGTCCATTG
	Rev: CAATGGACCAAAAGACGCCCGGTTTGTGAAGACAGAATA
F108P	Fwd: CTTCACAAACCGGAGGTCTGCTGGTCCATTGGGCGCTA
	Rev: TAGCGCCCAATGGACCAGCAGACCTCCGGTTTGTGAAG
S119A	Fwd: TATGAGAAACGCTCTCGCTCTGGCTGAGGATGAAG
	Rev: CTTCATCCTCAGCCAGAGCGAGAGCGTTTCTCATA
K212A	Fwd: CCCCTTTGTGGAAAACAGCAAGAAGCTCTTAGCCTTTAGTTTCTTTG
	Rev: CAAAGAAACTAAAGGCTAAGAGCTTCTTGCTGTTTTCCACAAAGGGG
F213A	Fwd: CCCCTTTGTGGAAAACAGCAAGAAGCTCTTAAAAGCGAGTTTCTTTG
	Rev: CAAAGAAACTCGCTTTTAAGAGCTTCTTGCTGTTTTCCACAAAGGGG
F215A	Fwd: GCAAGAAGCTCTTAAAATTTAGTGCCTTTGATCCATTCCTTCTCTC
	Rev: GAGAGAAGGAATGGATCAAAGGCACTAAATTTTAAGAGCTTCTTGC
R372A	Fwd: CCCAATTGCTGCTGCACTTGAGAGGGCCTGTAAG
	Rev: CTTACAGGCCCTCTCAAGTGCAGCAGCAATTGGG
E374A	Fwd: GCTAGACTTGCGAGGGCCTGTAAGAAGGATG
	Rev: CATCCTTCTTACAGGCCCTCGCAAGTCTAGC

Note: The mutated sites are underlined in bold and italic.